Annotations and Queries | Synapse Documentation

Annotations and Queries

Annotations are key-value pairs stored as metadata for Projects, Files, Folders, Tables and Views. Annotations help users search for and find data.

Annotations can be based on an existing ontology or controlled vocabulary, or can be created in an ad hoc manner and modified later as the metadata evolves. Annotations are a powerful tool used to systematically group and/or describe things (files or data, etc.) in Synapse.

For example, if you have uploaded a collection of alignment files in the BAM file format from an RNA-sequencing experiment, each representing a sample and experimental replicate, you can use annotations to surface this information in a structured way. Sometimes, users encode this information in file names, e.g., sampleA_conditionB.bam, which makes it “human-readable” but not searchable.

In this case, the annotations you may want to add might look like this:

Annotation example

How to Assign Annotations

Annotations are either added during upload or at a later date. Functionality for working with annotations is available in the command line client, the Python client, the R client, or from the web. Using the programmatic clients facilitates batch and automated population of annotations across many files. The web client can be used to bulk update many files using Views.

Add New Annotations and Modify Existing Annotations

To add or modify annotations annotations on Projects, Files, Folders, Tables or Views in the web client, click Tools in the upper right corner and Annotations.

Annotation web location

Use the + icon to add multiple values for a single key and the x icon to remove values.

Annotation filetools location

To add annotations on multiple files, refer to the In Practice article Managing Custom Metadata at Scale for a tutorial on leveraging Views for annotation management.

Command line

To add annotations on a new file during upload:

synapse store sampleA_conditionB.bam --parentId syn00123 --annotations '{"fileFormat":"bam", "assay":"rnaSeq"}'

To add annotations on an existing file:

synapse set-annotations --id syn00123 --annotations '{"fileFormat":"bam", "assay":"rnaSeq"}'


To add annotations on a new file during upload:

entity = File(path="sampleA_conditionB.bam",parent="syn00123")
entity.annotations = {"fileFormat":"bam", "assay":"rnaSeq"}

To modify annotations on an existing file:

entity = syn.get_annotations("syn123")

# set key 'fileFormat' to have value 'fastq'
entity['fileFormat'] = 'fastq'



To add annotations on a new file during upload:

entity <- File("sampleA_conditionB.bam", parent="syn00123")
entity <- synStore(entity, annotations=list(fileFormat = "bam", assay = "rnaSeq"))

To modify annotations on an existing file:

entity <- synGet("syn00123")

##### Modify annotations and PRESERVE existing annotations
existing_annots <- synGetAnnotations(entity)
synSetAnnotations(entity, annotations = c(existing_annots, list(fileType = "bam", assay = "rnaSeq")))

##### Modify annotations and REMOVE existing annotations
synSetAnnotations(entity, annotations = list(fileType = "bam", assay = "rnaSeq"))


Note: You will only be able to query the files you currently have permission to access.

Queries in Synapse look SQL-like and you can query any Table or View with <synId>.

SELECT * FROM <synId> WHERE <expression>

The section are the conditions for limiting a search. Every entity has properties useful for searching:

  • All entities (Projects, Files, Folders, Tables/Views, Docker containers): id, name, createdOn, createdBy, modifiedOn, modifiedBy, etag, type, parentId, benefactorId, projectId

  • Versionable entities (Files, Table/Views): currentVersion

  • Files only: dataFileHandleId

Files also have an contentMd5, contentSize and contentType as properties. These properties are not available in a View and are not searchable.

SELECT * FROM syn123456 WHERE "id" = 'syn00012'

For a complete list of example queries, see:

SQL Query Examples

Finding Files in a Specific Project

To find all files in a specific Project, create a View in the web client. For example, if you’d like to see all files in a Project, navigate to your project and then the Tables tab. From there, click Tables Tools and Add File View. Click Add container and Enter Synapse Id to create a tabluar file view that contains every file in the project, which you can now query. Importantly, if you want to later query on annotations, you must select Add All Annotations. For a more in-depth look at this feature, please read our articles on File Views.

Listing Files in a Specific Folder

To list the Files in a specific Folder, you need to know the synID of the Folder (for example syn1524884, which has data from TCGA related to melanoma). All entities in this Folder will have a parentId of syn1524884.

The function to find all Files in this Folder is called “getChildren”:

foo = list(syn.getChildren(parent='syn1524884', includeTypes=['file']))
foo <- as.list(synGetChildren(parent='syn1524884', includeTypes=list('file')))

Queries on Annotations

If annotations have been added to Files, they can be used to discover files of interest from View syn123456. For example, you can identify all Files annotated as bam files (fileFormat = bam) with the following query:

SELECT * FROM syn123456 WHERE "fileFormat"='bam'

Likewise, if you had put the RNA-Seq related files described in the section above into the project syn00123 with the described annotations, then you could find all of the files for conditionB and sampleA:

SELECT * FROM syn123456 WHERE "projectId"='syn00123' AND "specimenID"='sampleA_conditionB'

Lastly, you can query on a subset of entities that have a specific annotation. You can limit the annotations you want displayed as following.

SELECT specimenID,genomeBuild,fileFormat,platform FROM file WHERE "projectId"='syn00123' AND "specimenID"='sampleA_conditionB'

Reproducible queries can be constructed using one of the analytical clients (command line, Python, and R) and on the web client, query results can be displayed in a table on a wiki page.

In a project, from the wiki page click Wiki Tools in the upper right corner to Edit Project Wiki. Click Insert and choose Table: Query on Files/Folders. Enter your query in the box and click the Insert button. Once you save the wiki page, the results will be displayed as a table.

Command line
synapse query "SELECT specimenID,genomeBuild,fileFormat,platform FROM syn123456 WHERE \"specimenID\"='sampleA_conditionB'"
result = syn.tableQuery("SELECT specimenID,genomeBuild,fileFormat,platform FROM syn123456 WHERE \"specimenID\"='sampleA_conditionB'")
result = synTableQuery("SELECT specimenID,genomeBuild,fileFormat,platform FROM syn123456 WHERE \"specimenID\"='sampleA_conditionB'")

How to Download Based on Queries

You can download files in a folder using queries. Currently this feature is only available in the command line client. For example, if you want to download all files in a File View that has a synapse id of syn00123, use

synapse get -q "SELECT * FROM file WHERE parentId = 'syn00123'"


Single quotes in Synapse queries must be replaced by double quotes or two single quotes. In order to query for the chemicalStructure of 4'-chemical:

SELECT * FROM syn123 where "chemicalStructure" = '4"-chemical'
# OR
SELECT * FROM syn123 where "chemicalStructure" = '4''-chemical'

See Also

Downloading Data, Tables

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